Identification and antifungal susceptibility pattern of Candida species isolated from patients with nosocomial candiduria

Introduction: Nosocomial candiduria could be due to cystitis, pyelonephritis, or fungusball in the urinary tract system. Several reports have shown candidemia and upper urinarytract involvement as the complications of candiduria. The aim of this study was to assessnosocomial candiduria; identify Candida isolates and determine their drug susceptibilitypattern. Materials and Methods: Urine samples of 115 hospitalized patients werecollected during a period of five months. Candida species were isolated and identifiedusing conventional and molecular (PCR-RFLP) diagnostic methods. Antifungalsusceptibility profiles for amphotericin B and fluconazole were performed using brothmicrodilution method, based on the Clinical and Laboratory Standards Institute (CLSI)M27-A2 guideline. Results: Nosocomial candiduria was diagnosed in 5 (4.3%) patients.The isolated Candida species were identified as Candida albicans (n: 4) and C. glabrata(n: 2). Two strains of C. albicans, and C. glabrata were resistant to fluconazole.Conclusion: Similar to several reports, the results of this study show that C. albicans isthe main Candida species causing nosocomial candiduria and drug resistant Candidaspecies are causative agents of candiduria in hospitalized patients.